Employment is one of the most fundamental forms of human activity, the damages of which can cause job burnout and reduce quality of life. In recent years, dealing with this issue has been the main goal of most organizations. The present study aims to compare job burnout and quality of life among active and inactive clerks in one of Tehran universities. In the present causative comparative study, 100 clerks of the age range (mean and standard deviation 40.93 ±8.63) of the university which was chosen by access sampling. According to Sharkey's physical activity questionnaire, they were then divided into two active and inactive groups. Then, they filled out two Maslach job burnout and quality of life questionnaires. A multivariate variance analysis test was used with SPSS 20 for data analysis. The data and findings indicate that concerning job burnout and its subscales (Emotional Exhaustion, Depersonalization), physically inactive individuals had higher means; in contrast, people with more activity had higher means; also, physically active individuals showed higher mean scores in quality-of-life variable, in both physical health and mental health total scores and physical function, emotional wellbeing, social function, and general health subscales. Physically inactive individuals showed higher means concerning function disorder owing to physical health and function disorder owing to emotional health and pain. There was no significant difference concerning the subscale of energy/fatigue. Findings indicate that physical activity, as a strategy, improves the capacity of the clerks so they have a better quality of life and deal with job burnout. Thus, it is suggested that physical activity be used as a strategic plan to increase the clerks' efficiency.